Human/Rat M-Ras/R-Ras3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3606
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human M-Ras
Met1-Leu208 (Lys181Glu)
Accession # O14807
Met1-Leu208 (Lys181Glu)
Accession # O14807
Specificity
Detects human and rat M-Ras/R-Ras3 in Western blots. Does not detect recombinant human R-Ras or TC21 (R-Ras2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Rat M-Ras/R-Ras3 Antibody
Detection of Human and Rat M‑Ras/R‑Ras3 by Western Blot.
Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M-Ras/R-Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for M-Ras/R-Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Rat M-Ras/R-Ras3 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: L6 rat myoblast cell line
Sample: L6 rat myoblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-Ras/R-Ras3
Muscle Ras (M-Ras), also known as R-Ras3, is a small GTP-binding protein of the Ras superfamily of GTPases. M-Ras comprises an R-Ras subfamily with fellow members R-Ras and TC21 (R-Ras2). While highly homologous elsewhere, M-Ras differs at its carboxyl terminus from both R-Ras and TC21, suggesting this GTPase may interact with novel signaling effectors.
Alternate Names
MRas, R-RAS3
Gene Symbol
MRAS
UniProt
Additional M-Ras/R-Ras3 Products
Product Documents for Human/Rat M-Ras/R-Ras3 Antibody
Product Specific Notices for Human/Rat M-Ras/R-Ras3 Antibody
For research use only
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