Human RECK Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1734
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RECK
Gly27-Val934
Accession # O95980
Gly27-Val934
Accession # O95980
Specificity
Detects human RECK in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human RECK Antibody
Detection of Human RECK by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RECK at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human RECK Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human RECK, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human RECK, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RECK
Long Name
Reversion-inducing-cysteine-rich Protein with Kazal Motifs
Alternate Names
ST15
Gene Symbol
RECK
UniProt
Additional RECK Products
Product Documents for Human RECK Antibody
Product Specific Notices for Human RECK Antibody
For research use only
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