Human Rex-1/ZFP42 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3598
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Rex‑1/ZFP42
Ser2-Lys150
Accession # NP_777560
Ser2-Lys150
Accession # NP_777560
Specificity
Detects human Rex‑1/ZFP42 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human YYI is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Rex-1/ZFP42 Antibody
Rex‑1/ZFP42 in BG01V Human Stem Cells.
Rex-1/ZFP42 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Sheep Anti-Human Rex-1/ZFP24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Rex‑1/ZFP42 in NTera‑2 Human Cell Line.
Rex-1/ZFP42 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Rex-1/ZFP42 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Rex-1/ZFP42 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Rex-1
Sample: Recombinant Human Rex-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rex-1/ZFP42
Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity.
Long Name
Zinc Finger Protein 42
Alternate Names
Rex1, ZFP42
Gene Symbol
ZFP42
UniProt
Additional Rex-1/ZFP42 Products
Product Documents for Human Rex-1/ZFP42 Antibody
Product Specific Notices for Human Rex-1/ZFP42 Antibody
For research use only
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