Human Ribosomal Protein S6/RPS6 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM5436
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2B Clone # 522731
Product Specifications
Immunogen
E. coli-derived recombinant human Ribosomal Protein S6/RPS6
Met1-Lys249
Accession # P62753
Met1-Lys249
Accession # P62753
Specificity
Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. Detects human Ribosomal Protein S6/RPS6 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody
Ribosomal Protein S6/RPS6 in HeLa Human Cell Line.
Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Ribosomal Protein S6/RPS6 Biotinylated Monoclonal Antibody (Catalog # BAM5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ribosomal Protein S6/RPS6
40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.
Alternate Names
RPS6
Gene Symbol
RPS6
UniProt
Additional Ribosomal Protein S6/RPS6 Products
Product Documents for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody
Product Specific Notices for Human Ribosomal Protein S6/RPS6 Biotinylated Antibody
For research use only
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