Th17 differentiated human peripheral blood mononuclear cells (PBMCs, panel A) or resting PBMCs (panel B) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and Mouse Anti-Human RORa/NR1F1 PE-conjugated Monoclonal Antibody (Catalog # IC8924P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Detection of ROR alpha/NR1F1 in Jurkat cells by Flow Cytometry

Jurkat cells were stained with Mouse Anti-Human ROR alpha/NR1F1 PE-conjugated Monoclonal Antibody (Catalog # IC8924P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human ROR alpha/NR1F1 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Th17 differentiated human peripheral blood mononuclear cells (PBMCs) or Jurkat human acute T cell leukemia cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR alpha/NR1F1

RORa (Retinoid-related Orphan Receptor alpha) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression. Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.

Long Name

Retinoic Acid-Related Orphan Receptor alpha

Alternate Names

NR1F1, RORA, RZRA

Entrez Gene IDs

6095 (Human)

Gene Symbol

RORA

UniProt

P35398

Additional ROR alpha/NR1F1 Products

Product Documents for Human ROR alpha/NR1F1 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human ROR alpha/NR1F1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human ROR alpha/NR1F1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC8924P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ROR alpha/NR1F1 PE-conjugated Antibody

Detection of ROR alpha/NR1F1 in Th17 Differentiated Human PBMCs by Flow Cytometry.