Human RPA2 Antibody

Detection of Human RPA2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Saos-2 human osteosarcoma cell line, SH-SY5Y human neuroblastoma cell line, and human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5937) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for RPA2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human RPA2 by Simple Western^TM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for RPA2 at approximately 38 kDa (as indicated) using 25 µg/mL of Goat Anti-Human RPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5937) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of RPA2 in Human Tonsil.

RPA2 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Goat Anti-Human RPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5937) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.