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Human S100A10 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1698

R&D Systems, part of Bio-Techne
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AF1698
AF1698-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunoelectron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human S100A10

Specificity

Detects human S100A10 in direct ELISAs and Western blots. In direct ELISAs, greater than 60% cross-reactivity with recombinant mouse S100A10 and less than 2% cross-reactivity with recombinant human S100P is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human S100A10 Antibody

S100A10 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

S100A10 in Human Kidney Cancer Tissue.

S100A10 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 5 µg/mL Goat Anti-Human S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1698) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human S100A10 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human S100A10

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A10

S100A10 is a member of the S100 family of EF hand calcium-binding proteins. It is primarily found in mast cells as heterotetrameric complexes (two annexin A2 subunits and two S100 A10 subunits) that are present intracellularly at the plasma membrane or on the extracellular cell surface. Extracellular S100A10 is a key plasminogen receptor that plays an important role in cellular plasmin production and cellular invasiveness.

Long Name

S100 Calcium Binding Protein

Alternate Names

annexin II ligand, calpactin I, light polypeptide, ANX2L, ANX2LG, CAL1LGP11,42C, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, CLP11Ca[1], MGC111133, p10, p10 protein, p11, protein S100-A10, S100 calcium binding protein A10, S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)), S100 calcium-binding protein A10, S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))

Entrez Gene IDs

6281 (Human); 20194 (Mouse)

Gene Symbol

S100A10

Additional S100A10 Products

Product Documents for Human S100A10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human S100A10 Antibody

For research use only

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