Human S100A2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4870
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human S100A2
Met2-Pro98
Accession # P29034
Met2-Pro98
Accession # P29034
Specificity
Detects human S100A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, -A6, -A7, -A8, -A10, -A11, -A13, -A16, rhS100B and rhS100P is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human S100A2 Antibody
Detection of Human S100A2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human S100A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4870) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
S100A2 in MDA‑MB‑231 Human Cell Line.
S100A2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human S100A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4870) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human S100A2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MDA‑MB‑231 human breast cancer cell line
Sample: Immersion fixed MDA‑MB‑231 human breast cancer cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A2
S100A2 (also S100L and CaN19) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by multiple cell types including keratinocytes, chondrocytes, and bronchial epithelium. S100A2 regulates both Ca and Zn (in human) within cells and increases p53 activity. It forms both noncovalent and covalent homodimers and a homotetramer under certain conditions. Human S100A2 is 98 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 51‑86) plus a calcium (aa 64‑75) and zinc (aa 17‑22) binding site. There is one potential alternative splice form that shows a 40 aa insertion after Gly48. Full-length human S100A2 shares 89% and 85% aa identity with bovine and canine S100A2, respectively.
Long Name
S100 Calcium Binding Protein A2
Alternate Names
CAN19, S100L
Entrez Gene IDs
6273 (Human)
Gene Symbol
S100A2
UniProt
Additional S100A2 Products
Product Documents for Human S100A2 Antibody
Product Specific Notices for Human S100A2 Antibody
For research use only
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