Human S100A7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4475
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 577513
Product Specifications
Immunogen
E. coli-derived recombinant human S100A7
Met1-Gln101
Accession # P31151
Met1-Gln101
Accession # P31151
Specificity
Detects human S100A7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A8, A9, A10, A11, A14, A16, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human S100A7 Antibody
S100A7 in Human Skin.
S100A7 was detected in immersion fixed paraffin-embedded sections of human skin (hair follicle) using Mouse Anti-Human S100A7 Monoclonal Antibody (Catalog # MAB4475) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human S100A7 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin (hair follicle)
Sample: Immersion fixed paraffin-embedded sections of human skin (hair follicle)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A7
S100A7 (also Psoriasin) is an 11-13 kDa member of the S101 family, EF-hand superfamily of Ca-binding proteins. It is produced by keratinocytes and exists as both an intracellular and extracellular polypeptide. Intercellularly, it binds to E/FABP and may participate in fatty acid metabolism. Extracellularly, it is both monomeric and heterooligomeric and exhibits antibacterial properties. Human S100A7 is 101 amino acids (aa) in length. It contains two EF-hand motifs (aa 13-48 and 50-85), three zinc-binding His residues, one high-affinity Ca-binding site (aa 63-74) and an overlapping antibacterial sequence (aa 35-80). There appear to be no meaningful rodent structural orthologs. Human S100A7 is 93% aa identical to human S100A15/7A, and the S100A7 gene has a duplication (S100A7B) that is 54% aa identical to S100A7.
Long Name
S100 Calcium Binding Protein A7
Alternate Names
PSOR1
Gene Symbol
S100A7
UniProt
Additional S100A7 Products
Product Documents for Human S100A7 Antibody
Product Specific Notices for Human S100A7 Antibody
For research use only
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