Human SALM3/LRFN4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5445

R&D Systems, part of Bio-Techne
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MAB5445
MAB5445-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 578102

View all SALM3/LRFN4 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SALM3/LRFN4
Cys17-Leu518
Accession # Q6PJG9

Specificity

Detects human SALM3/LRFN4 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SALM1, approximately 20-25% cross-reactivity with recombinant human rhSALM2, and no cross-reactivity with rhSALM4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SALM3/LRFN4 Antibody

SALM3/LRFN4 antibody in Human Brain by Immunohistochemistry (IHC-P).

SALM3/LRFN4 in Human Brain.

SALM3/LRFN4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human SALM3/LRFN4 Monoclonal Antibody (Catalog # MAB5445) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SALM3/LRFN4 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM3/LRFN4

Synaptic adhesion-like molecule 3 (SALM3; also leucine-rich repeat and fibronectin type-III domain-containing protein 4 (LRFN4) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM3 is synthesized as a 635 amino acid (aa) precursor that contains a 16 aa signal sequence, a 502 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 96 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1 - 2).  In addition, there are six potential sites for N-linked glycosylation.  The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1 - 3, but is absent in SALMs 4 and 5 (3). Mature human SALM3 shares 97% aa sequence identity with mature mouse SALM3.  Northern blot analysis showed that in mice, SALM3 is strongly expressed in the adult brain and is also present in the adult testis (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM3, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).  SALM3 may also be involved in synapse formation, synaptic maintenance, and other cellular interactions (3).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Long Name

Synaptic Adhesion-like Molecule 3/Leucine-rich Repeat and Fibronectin Type III Domain Containing 4

Alternate Names

FIGLER6, LRFN4

Entrez Gene IDs

78999 (Human); 225875 (Mouse); 688721 (Rat)

Gene Symbol

LRFN4

UniProt

Q6PJG9 (Human)

Additional SALM3/LRFN4 Products

Product Documents for Human SALM3/LRFN4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SALM3/LRFN4 Antibody

For research use only

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