Human Semaphorin 4B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5485
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4B isoform 1
Leu39-Glu712
Accession # Q9NPR2
Leu39-Glu712
Accession # Q9NPR2
Specificity
Detects human Semaphorin 4B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Semaphorin 4B is observed and less than 1% cross‑reactivity with recombinant human (rh) Semaphorin 3A, 4A, and 7A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Semaphorin 4B Antibody
Detection of Human Semaphorin 4B by Western Blot.
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Semaphorin 4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5485) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Semaphorin 4B at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Semaphorin 4B in THP-1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Goat Anti-Human Semaphorin 4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5485, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).Applications for Human Semaphorin 4B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Western Blot
1 µg/mL
Sample: Human brain tissue
Sample: Human brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4B
Alternate Names
SEMA4B, SEMAC, SemC
Gene Symbol
SEMA4B
UniProt
Additional Semaphorin 4B Products
Product Documents for Human Semaphorin 4B Antibody
Product Specific Notices for Human Semaphorin 4B Antibody
For research use only
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