Human Semaphorin 4C Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6125
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 660016
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
Ala21-Gly663 (predicted)
Accession # Q9C0C4
Ala21-Gly663 (predicted)
Accession # Q9C0C4
Specificity
Detects human Semaphorin 4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4D, 4G, 5A, 5B, 6A, 6B, 6C,
6D, 7A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 4A, 4B, 4C, 4D, 4F, 4G, 6A,
6B, 6C, 6D, or 7A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Semaphorin 4C Antibody
Detection of Human Semaphorin 4C by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human Semaphorin 4C Monoclonal Antibody (Catalog # MAB6125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4C at approximately 100 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Semaphorin 4C Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4C
Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Semaphorin 4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499-551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21-663, human Semaphorin 4C shares 85% aa identity with mouse Semaphorin 4C.
Alternate Names
SEMA4C, SEMACL1, Semaf, SEMAI
Gene Symbol
SEMA4C
UniProt
Additional Semaphorin 4C Products
Product Documents for Human Semaphorin 4C Antibody
Product Specific Notices for Human Semaphorin 4C Antibody
For research use only
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