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Human Serpin B2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF8550

R&D Systems, part of Bio-Techne
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AF8550

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Knockout Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Serpin B2
Glu2-Pro415 (Asn66-Gln98 del)
Accession # P05120

Specificity

Detects human Serpin B2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) Serpin B6, rhSerpin B8, and rhSerpin B9 is observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Serpin B2 Antibody

Detection of Human Serpin B2 antibody by Western Blot.

Detection of Human Serpin B2 by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line and HEK001 human epidermal keratinocyte cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Serpin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8550) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Serpin B2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Serpin B2 antibody by Simple WesternTM.

Detection of Human Serpin B2 by Simple WesternTM.

Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for Serpin B2 at approximately 49 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Serpin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8550). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human Serpin B2 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Serpin B2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Seprin B2 knockout K562 cell line (KO) untreated (-) or treated (+) with 300nM PMA for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Serpin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8550) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Serpin B2 at approximately 40 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. HSP60 (Catalog # MAB1800) is shown as a loading control This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Serpin B2 Antibody

Application
Recommended Usage

Knockout Validated

Seprin B2 is specifically detected in K562 human chronic myelogenous leukemia parental cell line but is not detectable in Seprin B2 knockout K562 cell line.

Simple Western

1:100 dilution
Sample: U937 human histiocytic lymphoma cell line treated with PMA

Western Blot

1:1000 dilution
Sample: U937 human histiocytic lymphoma cell line and HEK001 human epidermal keratinocyte cell line treated with PMA

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Serpin B2

Serpin B2 is the gene that encodes plasminogen activator inhibitor 2 (PAI-2), a glycoprotein of 415 amino acids in length. The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases.

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Entrez Gene IDs

5055 (Human); 18788 (Mouse); 60325 (Rat)

Gene Symbol

SERPINB2

UniProt

Additional Serpin B2 Products

Product Documents for Human Serpin B2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Serpin B2 Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

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