Human Serpin B8/Proteinase Inhibitor 8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4158
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 423023
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # P50452
Asp2-Pro374
Accession # P50452
Specificity
Detects human Serpin B8/Proteinase Inhibitor 8 in direct ELISAs.
In direct ELISAs, approximately 25%
cross-reactivity with recombinant mouse (rm) Serpin B8 is observed. No
cross-reactivity with recombinant human Serpin A1, A3, A4, A5, A6, A9, A11,
A12, B3, B6, B9, C1, D1, E1, E2, F1, F2, G1, I1, I2, rmSerpin A1, A3N, A6, A9,
C1, D1, E1, E2, F1, F2, I1, recombinant rat (rr) Serpin A6, or rrSerpin A12 is
observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Serpin B8/Proteinase Inhibitor 8 Antibody
Serpin B8/Proteinase Inhibitor 8 in Human Pancreas.
Serpin B8/Proteinase Inhibitor 8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Human Serpin B8 Monoclonal Antibody (Catalog # MAB4158) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Serpin B8/Proteinase Inhibitor 8 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Reviewed Applications
Read 1 review rated 5 using MAB4158 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin B8/Proteinase Inhibitor 8
Serpin B8 (serine proteinase inhibitor-clade B #8; also PI8) is a 42‑45 kDa, cytoplasmic and secreted member of the ovalbumin (clade B)-subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types and shows inhibitory activity towards furin and other proteinases such as chymotrypsin. Human Serpin B8 is 374 amino acids (aa) in length. Although it has no signal sequence, it is released by platelets. The active inhibitor site lies between Arg336 and Arg342. One potential isoform shows a 2 aa substitution for the C-terminal 134 amino acids. Full length human Serpin B8 shares 78% and 83% aa identity with mouse and canine Serpin B8, respectively.
Alternate Names
CAP2, NK10, PI8
Gene Symbol
SERPINB8
UniProt
Additional Serpin B8/Proteinase Inhibitor 8 Products
Product Documents for Human Serpin B8/Proteinase Inhibitor 8 Antibody
Product Specific Notices for Human Serpin B8/Proteinase Inhibitor 8 Antibody
For research use only
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