Human Serpin D1/Heparin Cofactor II Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3198
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 373008
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
Gly20-Arg498
Accession # P05546
Gly20-Arg498
Accession # P05546
Specificity
Detects human Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human Serpin A3, A4, B6, B8, C1, I1, I2, recombinant mouse Serpin A1, A6, D1, E1, E2, F1, or F2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Serpin D1/Heparin Cofactor II Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)
Sample: Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)
Reviewed Applications
Read 4 reviews rated 3.8 using MAB3198 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin D1/Heparin Cofactor II
Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
- Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
Alternate Names
HC2, HCF2, Heparin Cofactor II, LS2
Gene Symbol
SERPIND1
UniProt
Additional Serpin D1/Heparin Cofactor II Products
Product Specific Notices for Human Serpin D1/Heparin Cofactor II Antibody
For research use only
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