Human SF20/MYDGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1147
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1009420
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SF20/MYDGF
Met1-Leu173
Accession # Q969H8
Met1-Leu173
Accession # Q969H8
Specificity
Detects human SF20/MYDGF in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human SF20/MYDGF Antibody
Detection of Human SF20/MYDGF by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, WI-38 human lung fibroblast cell line, human lung tissue, and human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SF20/MYDGF Monoclonal Antibody (Catalog # MAB1147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SF20/MYDGF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SF20/MYDGF on U937 Human Cell Line by Flow Cytometry.
U937 Human histiocytic lymphoma cell line was stained with Mouse Anti-Human SF20/MYDGF Monoclonal Antibody (Catalog # MAB1147, filled histogram) or Mouse IgG1 isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.Applications for Human SF20/MYDGF Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: U937 Human Histiocytic Lymphoma Cell Line fixed with paraformaldehyde and permeabilized with saponin
Sample: U937 Human Histiocytic Lymphoma Cell Line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
2 µg/mL
Sample: DU145 human prostate carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, WI‑38 human lung fibroblast cell line, Human lung tissue, and Human liver tissue
Sample: DU145 human prostate carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, WI‑38 human lung fibroblast cell line, Human lung tissue, and Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SF20/MYDGF
Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.
Long Name
Stromal Cell-derived Growth Factor SF20
Alternate Names
C19orf10, Ly6elg, MYDGF
Gene Symbol
MYDGF
UniProt
Additional SF20/MYDGF Products
Product Documents for Human SF20/MYDGF Antibody
Product Specific Notices for Human SF20/MYDGF Antibody
For research use only
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