Human SF20/MYDGF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1147
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SF20/MYDGF
Val32-Leu173
Accession # Q969H8
Val32-Leu173
Accession # Q969H8
Specificity
Detects human SF20/MYDGF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SF20/MYDGF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SF20/MYDGF Antibody
Detection of Human SF20/MYDGF by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Antibody (Catalog # AF1147) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SF20/MYDGF at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SF20/MYDGF by Simple WesternTM.
Simple Western lane view shows lysates of DU145 human prostate carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SF20/MYDGF at approximately 22 kDa (as indicated) using 25 µg/mL of Goat Anti-Human SF20/MYDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1147) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human SF20/MYDGF Antibody
Application
Recommended Usage
Simple Western
25 µg/mL
Sample: DU145 human prostate carcinoma cell line
Sample: DU145 human prostate carcinoma cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SF20/MYDGF
Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted) bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.
Long Name
Stromal Cell-derived Growth Factor SF20
Alternate Names
C19orf10, Ly6elg, MYDGF
Gene Symbol
MYDGF
UniProt
Additional SF20/MYDGF Products
Product Documents for Human SF20/MYDGF Antibody
Product Specific Notices for Human SF20/MYDGF Antibody
For research use only
Loading...
Loading...
Loading...
Loading...