Human SHP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1878
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 255402
Product Specifications
Immunogen
E. coli-derived recombinant human SHP-1
Ala205-Lys595
Accession # P29350
Ala205-Lys595
Accession # P29350
Specificity
Detects human SHP-1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human SHP-1 Antibody
SHP‑1 in HeLa Human Cell Line.
SHP-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human SHP-1 Monoclonal Antibody (Catalog # MAB1878) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human SHP-1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHP-1
Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).
References
- Tsui, H.W. et al. (1993) Nature Genet. 4:124.
- Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
- Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
- Pei, D. et al. (1994) Biochemistry 33:15483.
- Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.
Long Name
Src Homology Region 2 Domain Phosphatase 1
Alternate Names
HCPH, PTP1C, PTPN6, SHP1
Gene Symbol
PTPN6
UniProt
Additional SHP-1 Products
Product Documents for Human SHP-1 Antibody
Product Specific Notices for Human SHP-1 Antibody
For research use only
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