Human SIRP gamma/CD172g Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4486

R&D Systems, part of Bio-Techne
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MAB4486
MAB4486-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 595337

View all SIRP gamma/CD172g Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SIRP gamma/CD172g
Val64-Ser364
Accession # Q9P1W8

Specificity

Detects human SIRP gamma/CD172g in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant human (rh) SIRP alpha and rhSIRP beta 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SIRP gamma/CD172g Antibody

Detection of Human SIRP?/CD172g antibody by Western Blot.

Detection of Human SIRP gamma/CD172g by Western Blot.

Western blot shows lysates of NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP gamma/CD172g Monoclonal Antibody (Catalog # MAB4486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SIRP gamma/CD172g at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SIRP gamma/CD172g Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP gamma/CD172g

Signal-regulatory protein gamma/beta 2 (SIRP gamma/beta 2) is a 45-47 kDa type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily and SIRP family of proteins. Human SIRP gamma/beta 2 is synthesized as a 387 amino acid (aa) precursor that contains a 28 aa signal sequence, a 332 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 4 aa cytoplasmic tail. The ECD contains two Ig-like C1-type domains, one Ig-like V-type domain, and four potential sites for N-linked glycosylation. There are four named isoforms for human SIRP gamma/beta 2. Human SIRP gamma/beta 2 has no orthologs, but it is 75% aa identical to SIRP beta. It is expressed in the liver and at very low levels in brain, heart, lung, pancreas, kidney, placenta, and skeletal muscle. SIRP gamma/beta 2 is also expressed on CD4+ T-cells, CD8+ T-cells, and activated NK cells. 

Long Name

Signal-regulatory Protein gamma

Alternate Names

CD172g, SIRP beta 2, SIRPG

Entrez Gene IDs

55423 (Human); 102138723 (Cynomolgus Monkey)

Gene Symbol

SIRPG

UniProt

Q9P1W8

Additional SIRP gamma/CD172g Products

Product Documents for Human SIRP gamma/CD172g Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SIRP gamma/CD172g Antibody

For research use only

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