Human Sirtuin 2/SIRT2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4358
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 506611
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 2/SIRT2
Ala2-Gln389
Accession # Q8IXJ6
Ala2-Gln389
Accession # Q8IXJ6
Specificity
Detects human Sirtuin 2/SIRT2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SIRT1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Sirtuin 2/SIRT2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA), see our available Western blot detection antibodies
Sample: Cell lysates spiked with Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)
Sample: Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sirtuin 2/SIRT2
Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1, 2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3, 4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).
References
- Frye, R.A. (1999) Biochem. Biophys. Res. Commun. 260:273.
- North, B.J. et al. (2003) Mol. Cell. 11:437.
- North, B.J. and Verdin, E. (2004) Genome Biol. 5:224.
- Marmorstein, R. (2004) Biochem. Soc. Trans. 32:904.
- Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262:75.
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2L2, SIRT2
Gene Symbol
SIRT2
UniProt
Additional Sirtuin 2/SIRT2 Products
Product Documents for Human Sirtuin 2/SIRT2 Antibody
Product Specific Notices for Human Sirtuin 2/SIRT2 Antibody
For research use only
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