Human SIT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5016
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SIT1
Gln65-Ser196
Accession # Q9Y3P8
Gln65-Ser196
Accession # Q9Y3P8
Specificity
Detects human SIT1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human SIT1 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human SIT1
Sample: Recombinant Human SIT1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIT1
SIT1 (SHP-2-interacting transmembrane adapter 1; also signaling threshold-regulating transmembrane adapter 1) is a 35‑40 kDa type I transmembrane glycoprotein that belongs to a diverse group of transmembrane adapter proteins. It is expressed on thymocytes, plasma cells and T cells. When engaged, SIT1 downregulates TCR signaling. Mature human SIT1 is 172 amino acids (aa) in length. It contains a short extracellular domain (aa 25‑40) that undergoes homodimerization and an extensive intracellular region (aa 62‑196) that contains three key phosphorylation sites (Tyr90/169/188). Over aa 65‑196, human SIT1 is 86% and 80% aa identical to canine and mouse SIT1, respectively.
Long Name
Signaling Threshold Regulating Transmembrane Adaptor 1
Alternate Names
gp30/40
Entrez Gene IDs
27240 (Human)
Gene Symbol
SIT1
UniProt
Additional SIT1 Products
Product Documents for Human SIT1 Antibody
Product Specific Notices for Human SIT1 Antibody
For research use only
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