Human red blood cells were stained with Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of SLC14A1 in HEK293 Human Cell Line Transfected with Human SLC14A1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP was stained with either (A) Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human SLC14A1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human red blood cells and HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC14A1

Urea transporter 1, designated Solute Carrier family 14 member 1 (SLC14A1) is a 43-54 kDa multipass urea transporter mainly expressed in erythrocytes, but also found in kidney, mesenchymal stem cells, and some carcinomas. It contains an epitope identified as the Kidd (JK) blood group antigen. The antigenic forms differ at only one amino acid (aa); Asp280 represents Jk(a) and Asn280 represents Jk(b). SLC14A1 Isoform 2 (445 aa) contains an extended N-terminus as compared to Isoform 1 (389 aa). Human SLC14A1 shares 83% and 81% aa sequence identity with mouse and rat SLC14A1, respectively.

Long Name

Solute Carrier Family 14 (Urea Transporter), Member 1 (Kidd Blood Group

Alternate Names

HUT11, JK, RACH1, RACH2, UT-B1, UT1, UTE

Entrez Gene IDs

6563 (Human); 108052 (Mouse); 54301 (Rat)

Gene Symbol

SLC14A1

UniProt

Q13336

Additional SLC14A1 Products

Product Documents for Human SLC14A1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SLC14A1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SLC14A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8238

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SLC14A1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SLC14A1 Antibody

Detection of SLC14A1 in Human Red Blood Cells by Flow Cytometry.