Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLC22A1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection SLC22A1 in HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with either (A) human SLC22A1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A control antibody (IC003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human SLC22A1 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC22A1

SLC22A1 (solute carrier family 22 member 1), also called OCT1, is a 554 amino acid (aa), 70-80 kDa 12-transmembrane glycoprotein of the SLC22 family of polyspecific organic cation transporters (OCT). SLC22A1 is expressed in epithelial cells and some neurons, with detection on hepatocyte sinusoidal membranes and enterocyte basolateral membranes. It transports selected cations bidirectionally and is inhibited by non-transported cations. Human SLC22A1 shares 78% aa identity with mouse and rat SLC22A1. In humans, three inactive isoforms of 506, 483 and 353 aa, lack C-terminal exons 5, 5 and 6, or 3 and 6, respectively.

Long Name

Solute carrier family 22 member 1

Alternate Names

OCT1

Entrez Gene IDs

6580 (Human); 24904 (Rat)

Gene Symbol

SLC22A1

UniProt

NP_003048

Additional SLC22A1 Products

Product Documents for Human SLC22A1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SLC22A1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SLC22A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6469

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all SLC22A1 Antibodies »

Product Summary for Human SLC22A1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SLC22A1 Antibody

Detection of Human SLC22A1 by Western Blot.