Human SLC22A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6469
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 609222
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human SLC22A1
Met1-Thr554
Accession # NP_003048
Met1-Thr554
Accession # NP_003048
Specificity
Detects human SLC22A1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human SLC22A1 Antibody
Detection of Human SLC22A1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLC22A1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection SLC22A1 in HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP by Flow Cytometry
HEK293 human embryonic kidney cell line transfected with either (A) human SLC22A1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human SLC22A1 Monoclonal Antibody (Catalog # MAB6469) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A control antibody (IC003). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human SLC22A1 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human SLC22A1 and eGFP
Western Blot
2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLC22A1
SLC22A1 (solute carrier family 22 member 1), also called OCT1, is a 554 amino acid (aa), 70-80 kDa 12-transmembrane glycoprotein of the SLC22 family of polyspecific organic cation transporters (OCT). SLC22A1 is expressed in epithelial cells and some neurons, with detection on hepatocyte sinusoidal membranes and enterocyte basolateral membranes. It transports selected cations bidirectionally and is inhibited by non-transported cations. Human SLC22A1 shares 78% aa identity with mouse and rat SLC22A1. In humans, three inactive isoforms of 506, 483 and 353 aa, lack C-terminal exons 5, 5 and 6, or 3 and 6, respectively.
Long Name
Solute carrier family 22 member 1
Alternate Names
OCT1
Gene Symbol
SLC22A1
UniProt
Additional SLC22A1 Products
Product Documents for Human SLC22A1 Antibody
Product Specific Notices for Human SLC22A1 Antibody
For research use only
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