Human Smad1 Antibody

Detection of Human Smad1 by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad1 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Smad1 by Western Blot

Up-regulation of BMP signaling in the Fstl1-/- ureter and kidney.(A) Western blots of pSmad1/5/8, Smad1, pAKT (Ser473), AKT, and beta-actin from E15.5 (left panel) and E16.5 (second panel) ureter protein, E18.5 kidney protein (third panels), and HEK293 cells treated with the conditioned media containing BMP4 (20 ng/ml) transfected either with the Fstl1 or pcDNA3.1 vector (Mock) for 30 min (right panels). (B) Quantitative real-time PCR of Bmp2 (n = 5, p = 0.35), Bmp4 (n = 4, p = 0.73), Bmp5 (n = 5, p = 0.63), Bmp7 (n = 5, p = 0.50), TGF-beta 1 (n = 5, p = 0.51), Alk3 (n = 6, p = 0.40), Alk6 (n = 5, p = 0.21), Alk5 (n = 4, p = 0.47), BmprII (n = 6, p = 0.73), ActrIIa (n = 5, p = 0.97), and ActrIIb (n = 5, p = 0.73) from E16.5 ureters. (C) Co-immunoprecipitation of Myc-Fstl1 and HA-tagged BMP/TGF-beta receptors in HEK293 cells. Myc-Fstl1 can be immunoprecipitated with the anti-c-Myc antibody. Note that HA-ALK6 was co-immunoprecipitated by the anti-c-Myc and detected by the anti-HA antibody (lane 4). Scale bar: 40µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0032554), licensed under a CC-BY license. Not internally tested by R&D Systems.