Human Smad8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2309
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Smad8
Ala158-Ser233
Accession # NP_005896
Ala158-Ser233
Accession # NP_005896
Specificity
Detects human Smad8 in direct ELISAs and Western blots. In direct ELISAs, approximately 90% cross-reactivity with recombinant (rh) Smad1 is observed, approximately 20% cross-reactivity with rhSmad4 is observed, and less than 1% cross-reactivity with rhSmad5, rhSmad7, and rhDNAK is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Smad8 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast
Sample: Immersion fixed paraffin-embedded sections of human breast
Western Blot
0.1 µg/mL
Sample: Recombinant Human Smad8
Sample: Recombinant Human Smad8
Reviewed Applications
Read 2 reviews rated 4 using AF2309 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Smad8
Long Name
Mothers Against DPP Homolog 8
Alternate Names
Smad9
Entrez Gene IDs
4093 (Human)
Gene Symbol
SMAD9
UniProt
Additional Smad8 Products
Product Documents for Human Smad8 Antibody
Product Specific Notices for Human Smad8 Antibody
For research use only
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