Human SOCS-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4979

R&D Systems, part of Bio-Techne
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MAB4979
MAB4979-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 517206

View all SOCS-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOCS-2
Met1-Gln197 (predicted)
Accession # O14508

Specificity

Detects human SOCS-2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SOCS-2 Antibody

Detection of Human SOCS-2 antibody by Western Blot.

Detection of Human SOCS-2 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human SOCS-2 Monoclonal Antibody (Catalog # MAB4979) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for SOCS-2 was detected at approximately 23-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SOCS-2 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-2

SOCS-2 (Suppressor of cytokine signaling 2; also CIS-2 and SSI-2) is a 25 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by multiple cell types. It blocks receptor signaling by binding to phosphorylated tyrosines in the cytoplasmic domain of select receptors, including the receptors for leptin, IGF-I and GH. Human SOCS-2 is 198 amino acids (aa) in length. It contains one SH2 domain (aa 48‑156) and a SOCS box (aa 160‑198) that mediates interaction with elongin B or C of the E3 ubiquitin ligase complex. There is one splice variant that shows an Arg-rich eight aa substitution for aa 31‑39. Full-length SOCS-2 is 94% and 96% aa identical to mouse and porcine SOCS-2, respectively.

Long Name

Suppressor Of Cytokine Signaling 2

Alternate Names

CIS2, Cish2, SOCS2, SSI2, STATI2

Entrez Gene IDs

8835 (Human); 84607 (Rat)

Gene Symbol

SOCS2

UniProt

O14508

Additional SOCS-2 Products

Product Documents for Human SOCS-2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SOCS-2 Antibody

For research use only

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