Human SOCS-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4979
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOCS-2
Met1-Gln197
Accession # O14508
Met1-Gln197
Accession # O14508
Specificity
Detecs human SOCS-2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SOCS-2 Antibody
Detection of Human SOCS-2 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human SOCS-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for SOCS-2 was detected at approximately 23-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human SOCS-2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOCS-2
SOCS-2 (Suppressor of cytokine signaling 2; also CIS-2 and SSI-2) is a 25 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by multiple cell types. It blocks receptor signaling by binding to phosphorylated tyrosines in the cytoplasmic domain of select receptors, including the receptors for leptin, IGF-I and GH. Human SOCS-2 is 198 amino acids (aa) in length and contains one SH2 domain (aa 48‑156) and a SOCS box (aa 160‑198) that mediates interaction with elongin B or C of the E3 ubiquitin ligase complex. There is one splice variant that shows an Arg-rich eight aa substitution for aa 31‑39. Full-length SOCS-2 is 94% and 96% aa identical to mouse and porcine SOCS-2, respectively.
Long Name
Suppressor Of Cytokine Signaling 2
Alternate Names
CIS2, Cish2, SOCS2, SSI2, STATI2
Gene Symbol
SOCS2
UniProt
Additional SOCS-2 Products
Product Documents for Human SOCS-2 Antibody
Product Specific Notices for Human SOCS-2 Antibody
For research use only
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