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Human SOX13 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5118

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB5118-100
MAB5118-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2559A

Product Specifications

Immunogen

E. coli-derived recombinant human SOX13
Met19-Pro258
Accession # Q9UN79

Specificity

Detects human SOX13 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human SOX13 Antibody

SOX13 in Human Pancreas.

SOX13 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human SOX13 Monoclonal Antibody (Catalog # MAB5118) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei and cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human SOX13 Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX13

SOX-13 is a transcription factor involved in the embryonic development and the determination of cell fate.  It is also known as islet cell antibody 12 since it has been determined to be a type-1 diabetes autoantigen.  SOX-13 RNA is detected in most human tissues with highest levels in the pancreas, placenta, and kidney.  Immunohistochemistry on human pancreas identifies SOX-13 in the islets of Langerhans where staining is mostly cytoplasmic but can also be nuclear. SOX-13 antibody testing helps identify latent autoimmune diabetes of adults (LADA).

Long Name

SRY (sex determining region Y)-box 13

Alternate Names

ICA12

Entrez Gene IDs

9580 (Human); 20668 (Mouse); 289026 (Rat)

Gene Symbol

SOX13

UniProt

Additional SOX13 Products

Product Documents for Human SOX13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SOX13 Antibody

For research use only

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