Human Sp3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4256
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Sp3
Asp53-Ser309
Accession # Q02447
Asp53-Ser309
Accession # Q02447
Specificity
Detects human Sp3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Sp3 Antibody
Detection of Human Sp3 by Western Blot.
Western blot shows nuclear extracts of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sp3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Sp3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Sp3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sp3
Long Name
Sp3 Transcription Factor
Alternate Names
SPR-2
Gene Symbol
SP3
UniProt
Additional Sp3 Products
Product Documents for Human Sp3 Antibody
Product Specific Notices for Human Sp3 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...