Human SPRY1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6097
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 669534
Product Specifications
Immunogen
E.coli-derived recombinant human SPRY1
Met1-Lys178
Accession # O43609
Met1-Lys178
Accession # O43609
Specificity
Detects human SPRY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant
human SPRY2, 3, or 4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SPRY1 Antibody
Detection of Human SPRY1 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.5 µg/mL of Human SPRY1 Monoclonal Antibody (Catalog # MAB6097) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SPRY1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human SPRY1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: 293T human embryonic kidney cell line and LNCaP human prostate cancer cell line
Sample: 293T human embryonic kidney cell line and LNCaP human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SPRY1
SPRY1 (sprouty homolog 1) is a 34-38 kDa member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-gamma 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319 amino acids (aa) in length. It contains one CBL-TKB binding site (aa 51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa 112-131), and a Cys-rich domain (aa 181-306) that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa 1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.
Long Name
Sprouty Homolog 1
Alternate Names
Drosophila, homolog of, 1 (antagonist of FGF signaling), sprouty homolog 1, antagonist of FGF signaling (Drosophila), spry-1
Gene Symbol
SPRY1
UniProt
Additional SPRY1 Products
Product Documents for Human SPRY1 Antibody
Product Specific Notices for Human SPRY1 Antibody
For research use only
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