Human Synaptopodin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8977
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 918842
Product Specifications
Immunogen
E. coli-derived recombinant human Synaptopodin
Ser363-Gln490
Accession # Q8N3V7
Ser363-Gln490
Accession # Q8N3V7
Specificity
Detects human Synaptopodin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Synaptopodin Antibody
Synaptopodin in Human Kidney.
Synaptopodin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Synaptopodin Monoclonal Antibody (Catalog # MAB8977) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence
Double immunofluorescence staining of NLRC5 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRC5 (red) and CD31 (green). b Co-localization of NLRC5 (red) and integrin-alpha (green). c Co-localization of NLRC5 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Synaptopodin by Immunocytochemistry/Immunofluorescence
Double immunofluorescence staining of NLRP3 and glomerular intrinsic cells in AAV patients. a Co-localization of NLRP3 (red) and CD31 (green). b Co-localization of NLRP3 (red) and integrin-alpha (green). c Co-localization of NLRP3 (red) and synaptopodin (green). Scale bar = 50 μm in the bottom right Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186034), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Synaptopodin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Synaptopodin
Alternate Names
SYNPO
Gene Symbol
SYNPO
UniProt
Additional Synaptopodin Products
Product Documents for Human Synaptopodin Antibody
Product Specific Notices for Human Synaptopodin Antibody
For research use only
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