Human Syntaxin 12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6617
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 677201
Product Specifications
Immunogen
E. coli-derived recombinant human Syntaxin 12
Ser2-Gln200
Accession # Q86Y82
Ser2-Gln200
Accession # Q86Y82
Specificity
Detects human Syntaxin 12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity
with recombinant human Syntaxin 1A, 1B1, 1B2, 5, 6, 7, 8, or 16 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Syntaxin 12 Antibody
Detection of Human Syntaxin 12 by Western Blot.
Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line, LNCaP human prostate cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Syntaxin 12 Monoclonal Antibody (Catalog # MAB6617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 12 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Syntaxin 12 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: CCD‑1070Sk human foreskin fibroblast cell line, LNCaP human prostate cancer cell line, and MDA‑MB‑453 human breast cancer cell line
Sample: CCD‑1070Sk human foreskin fibroblast cell line, LNCaP human prostate cancer cell line, and MDA‑MB‑453 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syntaxin 12
Additional Syntaxin 12 Products
Product Documents for Human Syntaxin 12 Antibody
Product Specific Notices for Human Syntaxin 12 Antibody
For research use only
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