Human Syntaxin-BP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5900

R&D Systems, part of Bio-Techne
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AF5900
AF5900-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Syntaxin-BP2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin‑BP2
Glu81-Thr223
Accession # NP_001120868

Specificity

Detects human Syntaxin‑BP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Syntaxin-BP1 and rhSyntaxin-BP3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Syntaxin-BP2 Antibody

Detection of Human Syntaxin-BP2 antibody by Western Blot.

Detection of Human Syntaxin-BP2 by Western Blot.

Western blot shows lysates of human peripheral blood leukocyte. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin-BP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5900) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Syntaxin-BP2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human peripheral blood leukocyte

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntaxin-BP2

Syntaxin‑BP2 (STXBP2; Syntaxin-binding protein 2; also Unc18-2 and Unc18B) is a 64‑66 kDa member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is widely expressed, particularly in hematopoietic cells, and is found both in cytoplasm, and associated with membranes, where it binds to STX1A, 2, and 3. Thus, it presumably participates in primary granule exocytosis by promoting granule fusion. Human STXBP2 is 593 amino acids (aa) in length. It contains one SEC1 domain (aa 28‑576) with Trp28, Ser42, and Glu59 all playing a key role in syntaxin binding. There is one splice variant that shows a deletion of aa 83‑85. Over aa 80‑223, human and mouse STXBP2 share 95% aa sequence identity.

Long Name

Syntaxin-binding Protein 2

Alternate Names

STXBP2, SyntaxinBP2, Unc18-2, UNC18B

Entrez Gene IDs

6813 (Human); 20911 (Mouse); 81804 (Rat)

Gene Symbol

STXBP2

UniProt

NP_001120868

Additional Syntaxin-BP2 Products

Product Documents for Human Syntaxin-BP2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Syntaxin-BP2 Antibody

For research use only

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