Human Talin2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4738
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Talin2
Ala977-Arg1183
Accession # Q9Y4G6
Ala977-Arg1183
Accession # Q9Y4G6
Specificity
Detects endogenous human Talin2 in Western blots. In Western blots, no cross-reactivity to recombinant human Talin1 was detected.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Talin2 Antibody
Detection of Human Talin2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Talin2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Talin2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human Talin2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Talin2
Talin2 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 240 kDa cytoplasmic focal adhesion molecule. It likely forms dimers that link integrins to the actin cytoskeleton. Human Talin2 is 2542 amino acids (aa) in length. It contains one globular N‑terminal FERM domain that binds integrin cytodomains (aa 88‑406), followed by an Ala-rich region (aa 861‑1193) and a C‑terminal actin-binding domain (aa 2321‑2530). Human Talin2 has three potential variants. Two show alternate start sites at Met19 and Met1153, while a third has an alternate start site at Met1608 accompanied by a deletion of aa 1748‑1790. Over aa’s 977‑1183, human Talin2 shares 99% and 97% aa identity with canine and mouse Talin2, respectively.
Alternate Names
ILWEQ, TLN2
Gene Symbol
TLN2
UniProt
Additional Talin2 Products
Product Documents for Human Talin2 Antibody
Product Specific Notices for Human Talin2 Antibody
For research use only
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