Human TBX2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB50401
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 393720
Product Specifications
Immunogen
E. coli-derived recombinant human TBX2
Thr593-Arg702
Accession # Q13207
Thr593-Arg702
Accession # Q13207
Specificity
Detects human TBX2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX3, rhTBX6, or rhT‑bet is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human TBX2 Antibody
TBX2 in MCF‑7 Human Cell Line.
TBX2 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human TBX2 Monoclonal Antibody (Catalog # MAB50401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TBX2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TBX2
T-box protein 2 (TBX2) is a member of the T-box family of transcription factors and functions in the transcriptional regulation of genes required for mesoderm differentiation and limb bud pattern formation. The 74 kDa (predicted MW) nuclear protein, like other T-box family members, contains a T-domain which is involved in DNA binding and dimerization of the protein (aa 99‑277). Human TBX2 shows 96%, 95%, and 71% amino acid identity with dog, mouse, and Xenopus tropicalis TBX2, respectively.
Long Name
T-box Transcription Factor 2
Alternate Names
FLJ10169, T-box 2, T-box protein 2, T-box transcription factor TBX2
Gene Symbol
TBX2
UniProt
Additional TBX2 Products
Product Documents for Human TBX2 Antibody
Product Specific Notices for Human TBX2 Antibody
For research use only
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