Human TBX2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5040
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 393725
Product Specifications
Immunogen
E. coli-derived recombinant human TBX2
Thr593-Arg702
Accession # Q13207
Thr593-Arg702
Accession # Q13207
Specificity
Detects endogenous human TBX2 in Western blots at approximately 75 kDa.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human TBX2 Antibody
Detection of Human TBX2 by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, and BG0IV human embryonic stem cells. PVDF membrane was probed with 0.1 µg/mL of Human TBX2 Monoclonal Antibody (Catalog # MAB5040) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TBX2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TBX2 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: BG0IV human embryonic stem cells, HEK293 human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, and NTera-2 human testicular embryonic carcinoma cell line
Sample: BG0IV human embryonic stem cells, HEK293 human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, and NTera-2 human testicular embryonic carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TBX2
TBX2 (T-box transcription factor 2) is a member of a family of transcription factors that share the T-box DNA binding domain. TBX2, as well as other TBX proteins, is involved in remodeling of the heart myocardium through a complex spatial and temporal regulation. TBX2 is associated with tumorigenesis and anti-senescence properties by affecting several cyclin-dependent kinase inhibitors. The full-length protein contains 702 amino acids with a DNA binding domain from aa 99 to aa 277.
Long Name
T-box Transcription Factor 2
Alternate Names
FLJ10169, T-box 2, T-box protein 2, T-box transcription factor TBX2
Gene Symbol
TBX2
UniProt
Additional TBX2 Products
Product Documents for Human TBX2 Antibody
Product Specific Notices for Human TBX2 Antibody
For research use only
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