Human Tensin 4/CTEN Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6925
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 684524
Product Specifications
Immunogen
E. coli-derived recombinant human Tensin 4/CTEN
Ser2-Gln107
Accession # Q8IZW8
Ser2-Gln107
Accession # Q8IZW8
Specificity
Detects human Tensin 4/CTEN in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Tensin 4/CTEN Antibody
Detection of Human Tensin 4/CTEN by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tensin 4/CTEN at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Tensin 4/CTEN in Human Prostate.
Tensin 4/CTEN was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Tensin 4/ CTEN Monoclonal Antibody (Catalog # MAB6925) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Tensin 4/CTEN by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Tensin 4/CTEN at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Tensin 4/CTEN Monoclonal Antibody (Catalog # MAB6925). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Tensin 4/CTEN Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Simple Western
10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB6925 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tensin 4/CTEN
Alternate Names
CTEN, TNS4
Gene Symbol
TNS4
UniProt
Additional Tensin 4/CTEN Products
Product Documents for Human Tensin 4/CTEN Antibody
Product Specific Notices for Human Tensin 4/CTEN Antibody
For research use only
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