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Human Testican 1/SPOCK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2327

R&D Systems, part of Bio-Techne
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MAB2327
MAB2327-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 316801

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
Arg22-Trp439
Accession # Q08629

Specificity

Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Testican 2 or rhTestican 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Testican 1/SPOCK1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 1/SPOCK1

Testican 1, also known as SPOCK1, was originally identified in seminal plasma and is also expressed in brain. It is a proteoglycan that contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites as well as three potential inhibitory domains targeted toward three different classes of proteases, metallo-, cysteine and serine proteases. Human Testican 1 shares 95% amino acid sequence identity with mouse Testican 1.

Alternate Names

SPOCK1, TIC1, TICN1

Entrez Gene IDs

6695 (Human)

Gene Symbol

SPOCK1

UniProt

Additional Testican 1/SPOCK1 Products

Product Documents for Human Testican 1/SPOCK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Testican 1/SPOCK1 Antibody

For research use only

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