Human TFPI-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2545
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 243220
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TFPI-2
Asp23-Phe235
Accession # P48307
Asp23-Phe235
Accession # P48307
Specificity
Detects human TFPI-2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human TFPI-2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta
Sample: Immersion fixed paraffin-embedded sections of human placenta
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human TFPI‑2 (Catalog # 2545-PI) under non-reducing conditions only
Sample: Recombinant Human TFPI‑2 (Catalog # 2545-PI) under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TFPI-2
Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with an N-terminal acidic region, three Kunitz (K) domains (amino acids 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). It is a Kunitz-type serine protease inhibitor secreted by all cells of the vasculature (endothelial cells, smooth muscle cells, and fibroblasts) into the extracellular matrix (ECM), where it plays a role in the regulation of urokinase/plasmin-mediated ECM turnover. Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4).
References
- Miyagi, Y. et al. (1994) J. Biochem. 116:939.
- Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
- Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
- Rollin, J. et al. (2005) Br, J. Cancer 92:775.
Long Name
Tissue Factor Pathway Inhibitor 2
Alternate Names
PP5, REF-1, TFPI2
Entrez Gene IDs
7980 (Human)
Gene Symbol
TFPI2
UniProt
Additional TFPI-2 Products
Product Documents for Human TFPI-2 Antibody
Product Specific Notices for Human TFPI-2 Antibody
For research use only
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