Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB31202) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of TfR2 in K562 Human Cell Line by Flow Cytometry.

K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB31202, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human TfR2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: K562 human myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR2

Transferrin Receptor 2 (TfR2) is an iron-binding protein that is homologous to the transferrin receptor. Lack of active TfR2 has been associated with a rare form of hemochromatosis. The TfR2 alpha isoform is a type 2 transmembrane protein that is expressed in the liver and modulates hepcidin production in response to iron. It forms a 215 kDa disulfide-linked homodimer that is presumably glycated. The TfR2 beta isoform lacks the intracellular, transmembrane and part of the extracellular regions and is expressed ubiquitously. The extracellular portion of human TfR2 shares 86% aa identity with mouse.

Long Name

Transferrin Receptor 2

Alternate Names

HFE3, TFRC2

Entrez Gene IDs

7036 (Human); 50765 (Mouse)

Gene Symbol

TFR2

UniProt

Q9UP52

Additional TfR2 Products

Product Documents for Human TfR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TfR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TfR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB31202

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TfR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TfR2 Antibody

Detection of Human TfR2 by Western Blot.