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Human TGF-beta RII Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2411

R&D Systems, part of Bio-Techne
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MAB2411-100
MAB2411-500
MAB2411-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 176510

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TGF-beta  RII isoform 2
Thr23-Asp184
Accession # NP_001020018

Specificity

Detects human TGF-beta RII and TGF-beta RIIb in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TGF-beta RIII, recombinant mouse (rm) TGF-beta RII, or rmTGF-beta RI.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human TGF-beta RII Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta  RII (Catalog # 241-R2)
under non-reducing conditions only.
Human TGF-beta RII Affinity Purified Polyclonal Ab (Catalog # AF-241-NA)
is recommended for Western blot samples under reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

TGF-beta RII is a membrane-bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, containing a 25 amino acid residue insertion near the N-terminus of the mature protein has also been described.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Gene Symbol

TGFBR2

UniProt

Additional TGF-beta RII Products

Product Documents for Human TGF-beta RII Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TGF-beta RII Antibody

For research use only

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