Human Thimet Oligopeptidase/THOP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3439
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 455710
Product Summary for Human Thimet Oligopeptidase/THOP1 Antibody
Immunogen
E. coli-derived recombinant human Thimet Oligopeptidase/THOP1
Lys2-Cys689
Accession # P52888
Lys2-Cys689
Accession # P52888
Specificity
Detects human Thimet Oligopeptidase/THOP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human Neurolysin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Thimet Oligopeptidase/THOP1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN)
Sample: Recombinant Human Thimet Oligopeptidase/THOP1 (Catalog # 3439-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thimet Oligopeptidase/THOP1
Thimet Oligopeptidase/THOP1, also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1‑3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The amino acid sequence of human THOP1 is 90% and 86% identical to that of dog/mouse and rat, respectively.
References
- Barrett, A.J. and J.-M. Chen (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 352, Elsevier Academic Press, San Diego.
- Ray, K. et al. (2004) J. Biol. Chem. 279:20480.
- Saric, T. et al. (2004) J. Biol. Chem. 279:46723.
- Shivakumar, B.R. et al. (2005) Cell Biochem. Funct. 23:195.
Alternate Names
EP24.15
Gene Symbol
THOP1
UniProt
Additional Thimet Oligopeptidase/THOP1 Products
Product Documents for Human Thimet Oligopeptidase/THOP1 Antibody
Product Specific Notices for Human Thimet Oligopeptidase/THOP1 Antibody
For research use only
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