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Human THSD1 Isoform 2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5178

R&D Systems, part of Bio-Techne
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BAF5178

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human THSD1 Isoform 2
Glu25-Ile361
Accession # Q9NS62

Specificity

Detects human THSD1 Isoform 2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse THSD1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human THSD1 Isoform 2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human THSD1 Isoform 2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: THSD1

THSD1 (thrombospondin type 1 domain‑containing protein 1; also TMTSP) is a 90‑100 kDa type I transmembrane protein that is reminiscent of Unc5h proteins. It is expressed on embryonic endothelial and hematopoietic stem cells and may be involved in cell‑cell interactions. Mature human THSD1 is 827 amino acids (aa) in length. It contains a 388 aa extracellular domain (ECD) (aa 25‑412) and a 418 aa cytoplasmic region (aa 434‑851). The ECD possesses three Ig‑like domains
(aa 156‑316) and one TSP type‑1 domain (aa 340‑393). There is one potential alternate start site at Met380 and two splice variants, one of which shows a deletion of aa 341‑393 and a second that shows a 36 aa substitution for aa 394‑851, generating a soluble form. Over aa 25‑361, human THSD1 is 76% aa identical to mouse THSD1.

Long Name

Thrombospondin, Type I, Domain Containing 1

Alternate Names

TMTSP

Entrez Gene IDs

55901 (Human); 56229 (Mouse); 364630 (Rat)

Gene Symbol

THSD1

UniProt

Additional THSD1 Products

Product Documents for Human THSD1 Isoform 2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human THSD1 Isoform 2 Biotinylated Antibody

For research use only

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