Human Thyroglobulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8306
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 904802
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Thyroglobulin
Met1-Lys2768
Accession # P01266
Met1-Lys2768
Accession # P01266
Specificity
Detects human Thyroglobulin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Thyroglobulin Antibody
Detection of Human Thyroglobulin by Western Blot.
Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thyroglobulin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Thyroglobulin in Human Thyroid Cancer Tissue.
Thyroglobulin was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Thyroglobulin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thyroid cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human thyroid cancer tissue
Western Blot
1 µg/mL
Sample: Human thyroid tissue
Sample: Human thyroid tissue
Reviewed Applications
Read 1 review rated 4 using MAB8306 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thyroglobulin
Alternate Names
AITD3, cog, TDH3, Tg, TGN
Gene Symbol
TG
UniProt
Additional Thyroglobulin Products
Product Documents for Human Thyroglobulin Antibody
Product Specific Notices for Human Thyroglobulin Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...