HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 Mono-clonal Antibody (Catalog # MAB7998, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human TM4SF4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TM4SF4

TM4SF4 (transmembrane 4 L6 family member 4), also known as IL-TMP (intestine and liver tetraspanin membrane protein) is a member of the L6 tetraspanin family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia and periportal hepatocytes, with increased expression in hepatocellular carcinomas. It is thought to mediate proliferation and adhesion. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and 25-40 kDa for various glycosylated forms. The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. It shares 88% aa sequence identity with mouse and rat TM4SF4.

Long Name

Transmembrane 4 L Six Family Member 4

Alternate Names

ILTMP

Entrez Gene IDs

7104 (Human); 229302 (Mouse); 116467 (Rat)

Gene Symbol

TM4SF4

UniProt

P48230

Additional TM4SF4 Products

Product Documents for Human TM4SF4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TM4SF4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TM4SF4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7998

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TM4SF4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TM4SF4 Antibody

Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry.