Human TOB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5228
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TOB1
Met1-Asn345
Accession # P50616
Met1-Asn345
Accession # P50616
Specificity
Detects endogenous human TOB1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TOB1 Antibody
Detection of Human TOB1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Human TOB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5228) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TOB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human TOB1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF-7 human breast cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TOB1
TOB1 (transducer of ErbB2 protein 1) is a 45 kDa member of the BTG family of anti‑proliferative proteins. It is ubiquitously expressed, and serves to negatively regulate cell cycle progression from G0/G1 to S phase. This is accomplished by TOB1 suppression of cyclin D1 expression, which is abrogated by Erk2-mediated phosphorylation of Ser152/154/164 in response to growth factor stimulation. Human TOB1 is 345 amino acids (aa) in length and contains one NES (aa 2‑14), a bipartite NLS (aa 18‑40), a BTG domain (aa 42‑130) that possesses antiproliferative activity, and two PAM domains (aa 133‑143 and 268‑278) that bind inducible PABP and regulate gene transcription. Human and mouse TOB1 (aa 1‑345) are 94% aa identical when fourteen mouse-specific glutamines are excluded from the alignment.
Long Name
Transducer of ERBB2 Protein 1
Alternate Names
PIG49, TROB1
Gene Symbol
TOB1
UniProt
Additional TOB1 Products
Product Documents for Human TOB1 Antibody
Product Specific Notices for Human TOB1 Antibody
For research use only
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