Human TORC3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9455
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2069D
Product Specifications
Immunogen
E. coli-derived recombinant human TORC3
Ser425-Asn574
Accession # Q6UUV7
Ser425-Asn574
Accession # Q6UUV7
Specificity
Detects human TORC3 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human TORC3 Antibody
TORC3 in HeLa Human Cell Line.
TORC3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human TORC3 Monoclonal Antibody (Catalog # MAB9455) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human TORC3 Antibody
Application
Recommended Usage
Immunocytochemistry
1-10 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TORC3
CREB-Regulated Transcription Coactivator 3 (CRTC3), also known as TORC3, a transcriptional coactivator for CREB1, acts as a coactivator in the SIK/TORC signaling pathway. Dephosphorylated CRTC3 increases the interaction of CREB1 cAMP response element (CRE) sites. CRTC3 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1 transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1 transcription. CRTC3 polymorphism have been associated with obesity and hypertriglyceridemia.
Long Name
Transducer of Regulated CREB-binding Protein 3
Alternate Names
CRTC3
Gene Symbol
CRTC3
UniProt
Additional TORC3 Products
Product Documents for Human TORC3 Antibody
Product Specific Notices for Human TORC3 Antibody
For research use only
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