Human TPT1/TCTP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4648
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human TPT1/TCTP
Met1-Cys172
Accession # P13693
Met1-Cys172
Accession # P13693
Specificity
Detects human TPT1/TCTP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TPT1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human TPT1/TCTP Antibody
Detection of Human TPT1/TCTP by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TPT1/TCTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TPT1/TCTP at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human TPT1/TCTP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line and Nalm‑6 human Pre-B acute lymphocytic leukemia cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TPT1/TCTP
TPT-1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 22-23 kDa member of the TCTP family of calcium-binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator (Oct4), calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT-1 is 172 amino acids (aa) in length. It contains little in the way of structural motifs. There are multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99-172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1-172, human TPT-1 is 96% aa identical to mouse TPT-1.
Long Name
Translationally-controlled Tumor Protein 1
Alternate Names
Fortilin, HRF, p02, TCTP
Gene Symbol
TPT1
UniProt
Additional TPT1/TCTP Products
Product Documents for Human TPT1/TCTP Antibody
Product Specific Notices for Human TPT1/TCTP Antibody
For research use only
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