Human Transglutaminase 2/TGM2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4376
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 716620
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 2/TGM2
Ala2-Ala687 (Asn533Thr, Leu655Val)
Accession # P21980
Ala2-Ala687 (Asn533Thr, Leu655Val)
Accession # P21980
Specificity
Detects human Transglutaminase 2/TGM2 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human Transglutaminase 3, 4, 7, or recombinant mouse
Transglutaminase 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Transglutaminase 2/TGM2 Antibody
Detection of Human Transglutaminase 2/TGM2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Transglutaminase 2/TGM2 Monoclonal Antibody (Catalog # MAB4376) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transglutaminase 2/TGM2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Transglutaminase 2/TGM2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 2/TGM2
Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6‑7).
References
- Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
- Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
- Griffin, M. et al. (2002) Biochem. J. 368:377.
- Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
- Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
- Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
- Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Alternate Names
TGC, TGM2, Transglutaminase 2, tTG
Gene Symbol
TGM2
UniProt
Additional Transglutaminase 2/TGM2 Products
Product Documents for Human Transglutaminase 2/TGM2 Antibody
Product Specific Notices for Human Transglutaminase 2/TGM2 Antibody
For research use only
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