Human TRIM Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4708
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 488806
Product Specifications
Immunogen
E. coli-derived recombinant human TRIM
Thr50-Asn186
Accession # Q6PIZ9
Thr50-Asn186
Accession # Q6PIZ9
Specificity
Detects human TRIM in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TRIM Antibody
Detection of TRIM in Human CD3+Lymphocytes by Flow Cytometry.
Human blood-derived CD3+lymphocytes were stained with Mouse Anti-Human TRIM Monoclonal Antibody (Catalog # MAB4708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intra-cellular staining, cells were fixed with paraformaldehyde and permea-bilized with saponin.Applications for Human TRIM Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human blood-derived CD3+ lymphocytes fixed with paraformaldehyde and permeabilized with saponin
Sample: Human blood-derived CD3+ lymphocytes fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human TRIM
Sample: Recombinant Human TRIM
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRIM
Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30 kDa, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8 aa extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM.
Long Name
T Cell Receptor Interacting Molecule
Alternate Names
TCRIM, TRAT1
Gene Symbol
TRAT1
UniProt
Additional TRIM Products
Product Documents for Human TRIM Antibody
Product Specific Notices for Human TRIM Antibody
For research use only
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